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KMID : 1025520090510050413
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Journal of Animal Science and Technology
2009 Volume.51 No. 5 p.413 ~ p.420
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Effect of Gender-specific Bovine Serum Supplemented Medium on Cell Culture
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Lee Dong-Mok
Choi Moon-Seok
Woo Gyung-Il
Shin Yu-Mi
Lee Ki-Ho
Cheon Yong-Pil
Chun Tae-Hoon
Choi In-Ho
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Abstract
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The research has been aimed to investigate the effect of different sera including fetal bovine serum(FBS), male bovine serum (MS), female bovine serum(FS), and castrated-male bovine serum(C-MS) on cell proliferation, follicular maturation and ovulation in vitro. Established cell lines and primary cells were cultured in the culture media supplemented with different sera and cells proliferation was observed by cell counting and MTT assay. The results indicated that cell proliferation was significantly different for different serum source. Proliferation of bovine and human myogenic satellite cells was highest in MS. In contrast, proliferation of breast cancer cells and immune cells were the highest in FS and FBS, respectively. There was no difference in the rate of follicular growth, whereas the rate of ovulation was higher in FBS and C-MS. Finally, the wound healing effect and cell proliferation of 3T3-L1 cells showed that wound healing was fastest in FS and cell proliferation was higher in MS. These results suggest the importance of an optimal serum selection in the experiments involving cell culture system, and gender-specific Hanwoo sera may be used as a substitute to FBS.
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KEYWORD
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Bovine serum, Proliferation, MTT assay
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